Resumen de Auto-phylo: a pipeline maker for phylogenetic studies

Hugo López Fernández , Miguel Pinto, Cristina P. Vieira, Pedro Duque, Miguel Reboiro Jato , Jorge Vieira

• Inferences on the evolutionary history of a gene can provide insight into whether the findings made for a given gene in a given species can be extrapolated to other species, including humans, help explain morphological evolution or give an explanation for unexpected findings regarding gene expression suppression experiments, among others. The large amount of sequence data that is already available, and that is predicted to dramatically increase in the next few years, means that life science researchers need efficient automated ways of analyzing such data. Moreover, especially when dealing with divergent sequences, inferences can be affected by the chosen alignment and tree building algorithms, and thus the same dataset should be analyzed in different ways, reinforcing the need for the availability of efficient automated ways of analyzing the sequencing data. Therefore, here, we present auto-phylo, a simple pipeline maker for phylogenetic studies, and provide two examples of its utility: one involving a small already formatted sequenced dataset (41 CDS) to determine the impact of the use of different alignment and tree building algorithms in an automated way, and another one involving the automated identification and processing of the sequences of interest, starting from 16550 bacterial CDS FASTA files downloaded from the NCBI Assembly RefSeq database, and subsequent alignment and tree building inferences.