Effect of nitrate and irradiance on fatty acid production in microalgae cultivated for feeding larvae and broodstock conditioning in batch culture

• Fidelina González ^[1] ; Katia Sáez ^[1] ; María Eliana Vega ^[2] ; Berenice Bizama ; José Becerra ; Irene Lépez ; Víctor Hernández ^[1] ; Mario Silva
  1. [1] Universidad de Concepción

     Universidad de Concepción

     Comuna de Concepción, Chile

     
  2. [2] Consultoría Ambiental en Empresa Independiente
• Localización: Revista de biología marina y oceanografía, ISSN 0718-1957, ISSN-e 0717-3326, Vol. 54, Nº. 1, 2019, págs. 91-106
• Idioma: inglés
• DOI: 10.22370/rbmo.2019.54.1.1569
• Títulos paralelos:
  • Efectos de nitrato e irradiación en la producción de ácidos grasos en microalgas destinadas a la alimentación de larvas y reproductores, en cultivo experimental cerrado
• Enlaces
  • Texto completo (pdf)
• Dialnet Métricas: 1 Cita
• Resumen
  • español

    El objetivo de este estudio fue comparar las condiciones para producir altos niveles de AGPI, EPA y DHA en microalgas cultivadas para alimentar las etapas larvarias de los bivalvos. Se evaluó por cromatografía de gas la concentración de ácidos grasos poliinsaturados en tres especies de microalgas, Isochrysis galbana Parke (T-ISO), Chaetoceros gracilis Pantocsek y C. constrictus Gran, con el fin de ser usadas como fuente de alimento de larvas y adultos de bivalvos en cultivo. Isochrysis galbana produce mayor concentración de ácido linolénico y ácido docosahexaenoico que ácido eicosapentaenoico en condiciones limitantes de nitrato, sin embargo estos ácidos grasos se incrementan notablemente con el exceso de N-nitrato, por encima del contenido del medio F/2. Por otra parte, la baja intensidad lumínica y la concentración normal de nitratos en los cultivos de la especie Chaetoceros gracilis producen mayor contenido de ácido eicosapentaenoico (EPA). En el caso de Chaetoceros constrictus, la microalga sintetiza mayor concentración de ácidos grasos en condiciones de alta intensidad lumínica y bajas concentraciones de nitrato.

  • English

    The aim of this study was to compare conditions for the production of high levels of PUFA, EPA and DHA in cultured microalgae for larval bivalve feeding stages. It assessed fatty acid composition by gas chromatography for Isochrysis galbana Parke (T-ISO) and 2 species of Chaetoceros, C. gracilis Pantocsek and C. constrictus Gran. Species were studied in batch cultures, at the beginning of stationary growth phase with different irradiance and 2 levels of nitrogen content for Chaetoceros sp., and constant irradiance with 5 concentrations of sodium nitrate for I. galbana in an F/2 medium. Isochrysis galbana produced higher levels of linolenic acid and docosahexaenoic acid than eicosapentaenoic acid (EPA) at low N-nitrate concentrations; however, fatty acids increased notably with surplus N-nitrate, above F/2 medium content. Low irradiance and normal concentration of nitrates in the batches of Chaetoceros gracilis species produced a greater EPA content. C. constrictus, a diatom isolated from phytoplankton samples, presented higher levels of EPA and DHA at low levels of nitrogen and low irradiance.

• Referencias bibliográficas
  • Adarme-Vega, T.C.. (2012). Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production. Microbial Cell...
  • Bascur, M.. (2017). Seasonal changes in the biochemical composition of females and offspring of red squat lobster, Pleuroncodes monodon (Decapoda,...
  • Bell, M.V.. (1986). The role of polyunsaturated fatty acids in fish. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry....
  • Bellou, S.. (2014). Microalgal lipids biochemistry and biotechnological perspectives. Biotechnology Advances. 32. 1476
  • Benavente-Valdés, J.R.. (2016). Strategies to enhance the production of photosynthetic pigments and lipids in Chlorophycae species. Biotechnology...
  • Bonacic, K.. (2016). Mechanisms of lipid metabolism and transport underlying superior performance of Senegalese sole (Solea senegalensis,...
  • Brett, M.. (1997). The role of highly unsaturated fatty acids in aquatic foodweb processes. Freshwater Biology. 38. 483
  • Breuer, G.. (2012). The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource...
  • Caporgno, M.P.. (2018). Trends in microalgae incorporation into innovative food products with potential health benefits. Frontiers in Nutrition....
  • Castro, L.R.. (2010). Fatty acids in eggs of anchoveta Engraulis ringens during two contrasting winter spawning seasons. Marine Ecology Progress...
  • Cerpa, J.. (2003). Lipid and polypeptide profiles in the female portion of gonads from diet conditioned broodstock of North Chilean Scallops...
  • Chen, C.Y.. (2015). Improving protein production of indigenous microalga Chlorella vulgaris FSP-E by photobioreactor design and cultivation...
  • Cohen, Z.. (2000). The role of triacylglycerol as a reservoir of polyunsaturated fatty acids for the rapid production of chloroplastic lipids...
  • Colombo, S.M.. (2018). Potential for novel production of omega-3 long-chain fatty acids by genetically engineered oilseed plants to alter...
  • De la Peña, M.R.. (2007). Cell growth and nutritive value of the tropical benthic diatom, Amphora sp., at varying levels of nutrients and...
  • Di Rienzo, J.A.,Casanoves, F.,Balzarini, M.,Gonzalez, L.,Tablada, M.,Robledo, C.W.. (2015). InfoStat versión 2014. Grupo InfoStat, FCA, Universidad...
  • Domergue, F.. (2003). New insight into Phaeodactylum tricornutum fatty acid metabolism. Cloning and functional characterization of plastidial...
  • El Menyawi, I.. (2000). Identification of yeast fatty acid profiling as measured by gas-liquid chromatography. Journal of Chromatography....
  • Fakhrina, M.N.. (2018). Production of tropical oyster seed in hatchery. Survey in Fisheries Sciences. 5. 7-19
  • Fang, J.. (2004). Biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria. Comparative Biochemistry and Physiology...
  • Farias, A.. (2003). Polyunsaturated fatty acids in total lipid and phospholipids of Chilean scallop Argopecten purpuratus (L.) larvae: effects...
  • Feng, D.. (2011). Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement....
  • Folch, J.. (1957). A simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry....
  • Galloway, A.W.. (2015). Partitioning the relative importance of phylogeny and environmental conditions on phytoplankton fatty acids. PLoS...
  • Gordillo, F.J.. (1998). Effects of light intensity, CO2 and nitrogen supply on lipid class composition of Dunaliella viridis. Journal of Applied...
  • Grima, E.M.. (1992). EPA from Isochrysis galbana. Growth conditions and productivity. Process Biochemistry. 27. 299-305
  • González, H.E.. (1987). Desarrollo de dominancia local en la taxocenosis de fitoplancton de Bahía de Concepción, Chile, durante un evento...
  • Guevara, M.. (2016). Comparison of growth and biochemical parameters of two strains of Rhodomonas salina (Cryptophyceae) cultivated under...
  • Guschina, I.A.. (2009). Algal lipids and effect of the environment on their biochemistry. Lipids in aquatic ecosystems. 1-24
  • Hamre, K.. (2013). Fish larval nutrition and feed formulation: knowledge gaps and bottlenecks for advances in larval rearing. Reviews in Aquaculture....
  • Haro, P.. (2017). Physiological plasticity of a Chilean strain of the diatom Phaeodactylum tricornutum: the effect of culture conditions on...
  • Hemaiswarya, S.. (2011). Microalgae: a sustainable feed source for aquaculture. World Journal of Microbiology and Biotechnology. 27. 1737
  • Ho, S.Y.. (2007). Polyunsaturated fatty acids (PUFAs) content of the fungus Mortierella alpina isolated from soil. Journal of Agricultural...
  • Hu, Q.. (2008). Microalgal triacylglycerols as feedstocks for biofuel production: Perspectives and advances. The Plant Journal. 54. 621
  • Huerlimann, R.. (2014). The effect of nitrogen limitation on acetyl-CoA carboxylase expression and fatty acid content in Chromera velia and...
  • Kim, G.. (2016). . Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid...
  • Khoeyi, Z.A.. (2012). Effect of light intensity and photoperiod on biomass and fatty acid composition of the microalgae, Chlorella vulgaris....
  • Liang, Y.. (2006). Changes in growth, chlorophyll fluorescence and fatty acid composition with culture age in batch cultures of Phaeodactylum...
  • Lourenço, S.O.. (2002). Effects of different nitrogen sources on the growth and biochemical profile of 10 marine microalgae in batch culture:...
  • Lowry, O.H.. (1951). Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry. 193. 265
  • Madkour, F.F.. (2012). Production and nutritive value of Spirulina platensis in reduced cost media. The Egyptian Journal of Aquatic Research....
  • Metcalfe, L.D.. (1966). Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analytical Chemistry. 38. 514
  • Minhas, A.K.. (2016). A review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids. Frontiers...
  • Miller, M.R.. (2014). Changes in oil content, lipid class and fatty acid composition of the microalga Chaetoceros calcitrans over different...
  • Monroig, Ó.. (2013). Biosynthesis of polyunsaturated fatty acids in marine invertebrates: recent advances in molecular mechanisms. Marine...
  • Mortensen, S.H.. (1988). Fatty acid and elemental composition of the marine diatom Chaetoceros gracilis Schütt. Effects of silicate deprivation,...
  • Mühlroth, A.. (2013). Pathways of lipid metabolism in marine algae, co-expression network, bottlenecks and candidate genes for enhanced production...
  • Napolitano, G.E.. (1990). Fatty acid composition of three cultured algal species (Isochrysis galbana, Chaetoceros gracilis and Chaetoceros...
  • Napolitano, G.E.. (1997). Fatty acids as trophic markers of phytoplankton blooms in the Bahia Blanca Estuary (Buenos Aires, Argentina) and...
  • Pettersen, A.K.. (2010). Effects of different dietary microalgae on survival, growth, settlement and fatty acid composition of blue mussel...
  • Ragg, N.L.. (2010). Optimising the delivery of the key dietary diatom Chaetoceros calcitrans to intensively cultured Greenshell™ mussel larvae,...
  • Ramírez, J.J. (1982). El fitoplancton: métodos de muestreo, concentración, recuento y conservación. Actualidades Biológicas. 11. 30
  • Renaud, S.M.. (2002). Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown...
  • Rico-Villa, B.. (2006). Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific...
  • Rivera, P.. (1969). Sinopsis de las diatomeas de la Bahía de Concepción, Chile. Gayana Botanica. 1-112
  • Roopnarain, A.. (2015). Time of culture harvest affects lipid productivity of nitrogen-starved Isochrysis galbana U4 (Isochrysidales, Haptophyta)....
  • Ryckebosch, E.. (2013). Stability of omega-3 LC-PUFA-rich photoautotrophic microalgal oils compared to commercially available omega-3 LC-PUFA...
  • Ryckebosch, E.. (2014). Nutritional evaluation of microalgae oils rich in omega-3 long chain polyunsaturated fatty acids as an alternative...
  • Ryckebosch, E.. (2014). Influence of extraction solvent system on the extractability of lipid components from the biomass of Nannochloropsis...
  • Santos, A.M.. (2012). Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions. Bioresource Technology....
  • Sasaki, T.. (2006). Correlation between thermotolerance and membrane properties in Paramecium aurelia. Journal of Experimental Biology. 209....
  • Solovchenko, A.E. (2008). Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green...
  • Sukenik, A.. (1991). Variations in lipid and fatty acid content in relation to acetyl CoA carboxylase in the marine prymnesiophyte Isochrysis...
  • Tocher, D.R.. (2015). Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective. Aquaculture. 94-107
  • Van Wagenen, J.. (2012). Effects of light and temperature on fatty acid production in Nannochloropsis salina. Energies. 5. 731
  • Velasco, L.A.. (2016). Isolation, culture and evaluation of Chaetoceros muelleri from the Caribbean as food for the native scallops, Argopecten...
  • Yodsuwan, N.. (2017). Effect of nitrogen concentration on growth, lipid production and fatty acid profiles of the marine diatom Phaeodactylum...
  • Yu, W.L.. (2011). Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae. Microbial Cell Factories....
  • Zhang, Y.M.. (2013). Nitrogen starvation induced oxidative stress in an oil-producing green alga Chlorella sorokiniana C3. PloS One. 8.