Evaluation of the Antimicrobial Activity of Chitosan Biofilms Modified with Silver Nanoparticles

• Valverde-Durán, Sergio ^[1] ; Ramírez-Sarmiento, Angie ^[1] ; Ruilova-Tigre, Dennis ^[1] ; González-Carrasco, Víctor ^[1] ; Manzanares-Loaiza, Silvana ^[1] ; Debut, Alexis ^[2]
  1. [1] Universidad Tecnica de Machala

     Universidad Tecnica de Machala

     Machala, Ecuador

     
  2. [2] Escuela Politécnica del Ejército

     Escuela Politécnica del Ejército

     Sangolqui, Ecuador

     
• Localización: Revista Politécnica, ISSN-e 2477-8990, Vol. 56, Nº. 1, 2025 (Ejemplar dedicado a: Revista Politécnica), págs. 37-46
• Idioma: inglés
• DOI: 10.33333/rp.vol56n1.03
• Títulos paralelos:
  • Evaluación de la Actividad Antimicrobiana de Biopelículas de Quitosano Modificadas con Nanopartículas de Plata
• Enlaces
  • Texto completo
• Resumen
  • español

    La resistencia a los antibióticos es reconocida como un grave problema de salud global, con tasas crecientes de morbilidad y mortalidad cada año. Como consecuencia, a lo largo de los años se han desarrollado biomateriales innovadores para combatir la proliferación bacteriana, centrándose especialmente en biopelículas modificadas con nanopartículas metálicas. Para este trabajo, en la fase inicial, se sintetizaron nanopartículas de plata (AgNPs) a niveles de pH de 10.9, 11.5 y 12 mediante el método de coprecipitación. Posteriormente, las biopelículas de quitosano fueron sintetizadas químicamente mediante el método sol-gel, lo que permitió su modificación mediante la incorporación de AgNPs en concentraciones de 0, 0.25, 0.5 y 0.75 %. La evaluación antimicrobiana se realizó contra E. coli, revelando que la adición de AgNPs a las biopelículas de quitosano mejoró la actividad antimicrobiana. Se observaron los mayores diámetros de inhibición en las biopelículas que contenían 0.75 % de AgNPs a pH 10.9, alcanzando un halo máximo de inhibición de 11.93 mm. Los materiales sintetizados fueron caracterizados mediante FTIR, XRD y SEM.

  • English

    Antibiotic resistance is recognized as a severe global health issue, with increasing morbidity and mortality rates each year. Consequently, innovative biomaterials have been developed over the years to combat bacterial proliferation, particularly focusing on biofilms modified with metallic nanoparticles. For this work in the initial phase, silver nanoparticles (AgNPs) were synthesized at pH levels of 10.9, 11.5, and 12 using the coprecipitation method. Subsequently, chitosan biofilms were chemically synthesized via the sol-gel method, which allowed for the modification of these biofilms through the incorporation of AgNPs at concentrations of 0, 0.25, 0.5, and 0.75 %. Antimicrobial evaluation was performed against E. coli, revealing that the addition of AgNPs to chitosan biofilms enhanced antimicrobial activity, with the greatest inhibition diameters observed in biofilms containing 0.75 % AgNPs at pH 10.9, achieving a maximum inhibition halo of 11.93 mm. The synthesized materials were characterized using FTIR, XRD, and SEM.

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