Comparación de métodos que utilizan ácido sulfúrico para la determinación de azúcares totales

• Xiomara López-Legarda ^[1] ; Andony Taramuel-Gallardo ^[1] ; Carolina Arboleda-Echavarría ^[1] ; Freimar Segura-Sánchez ^[1] ; Luis Fernando Restrepo-Betancur ^[1]
  1. [1] Universidad de Antioquia

     Universidad de Antioquia

     Colombia

     
• Localización: Revista Cubana de Química, ISSN-e 2224-5421, Vol. 29, Nº. 2, 2017, págs. 180-198
• Idioma: español
• Títulos paralelos:
  • Comparison of methods using sulfuric acid for determination of total sugars
• Enlaces
  • Texto completo
• Dialnet Métricas: 1 Cita
• Resumen
  • español

    Este artículo presenta una comparación entre el método fenol-ácido sulfúrico y dos variaciones de este, las cuales consisten en cambiar el orden de adición de reactivos y la eliminación del fenol. Se realizaron varias curvas de calibración comparando las pendientes obtenidas de la ecuación de la recta. Se confirmó que la adición de fenol no es necesaria para la cuantificación de carbohidratos totales. Se evaluó la capacidad del método para cuantificar diferentes azúcares (glucosa, xilosa, galactosa y 1,3-ß-glucano), la estabilidad de la reacción en el tiempo, la necesidad de agitación y la reducción del volumen de reacción; además se realizó la validación de la curva de calibración propuesta. Se logró concluir que el método no requiere el uso de fenol y que se puede reducir el volumen final de la reacción de 8 mL hasta 1,3 mL conservando la reproducibilidad y disminuyendo costos de reactivos y cantidad de residuos.

  • English

    This article presents a comparison between phenol-sulfuric acid method and two variations, that propose to vary the order of reagents addition or remove phenol reaction. It was performed several calibration curves to compare the slopes obtained from the straight-line equation. It was confirmed that the addition of phenol is not necessary for the quantification of total carbohydrates. The ability of the method to quantify different sugars (glucose, xylose, galactose and 1,3-ß-glucan), the stability of the reaction, the agitation and the reaction volume were evaluated. A validation of calibration curve was made. It was concluded that the method for the determination of total sugars does not require the use of phenol and can reduce the final reaction volume of 8 mL to 1,3 mL. This research has a positive impact in order to reduce costs of reagents and the amount of wastes.

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